Next week, Monday 13 and Tuesday 14 October, the e-Cosmetic 360 online trade show will take place. For the occasion, we will present our new in-vitro efficacy tests on cell and tissue models.

Since the last time we participated in this event dedicated to innovation, we have developed 5 tests focusing on 2 main themes: skin inflammation and cellular senescence in relation to skin ageing and one of its consequences, a dulled complexion

SKIN INFLAMMATION

For this theme, two efficacy tests were designed for dermocosmetic active ingredients and finished products.

• The first model targets inflammation and itching mechanisms (pruritis) in the context of allergies. This in-vitro test, “designed” on reconstructed epidermis, assesses the capability of ingredients and finished products to regulate the skin’s reaction following exposure to pruritogens, by monitoring specific markers.

• The second model targets allergy mechanisms. This test was designed in 2 parts targeting keratinocyte (HaCaT) and mastocyte (MC/9) cells. It tests the capability of an ingredient to regulate the skin’s reaction to allergy / inflammation stress by monitoring specific markers, especially histamine and certain cytokines.

CELLULAR SENESCENCE & SKIN AGEING

For this theme, several efficacy tests were developed for dermocosmetic active ingredients and finished products.

• The first model targets cellular senescence mechanisms. This test was designed using a cellular model: normal human dermal fibroblasts (NHDF) maintained in a premature senescence state. By monitoring the markers of the senolytic state, beta-galactosidase and P21, as well as structural and inflammatory markers, this new test assesses the capability of ingredients to regulate the state of cellular senescence.

• The second model assesses the biological consequences related to “dulled complexion” following vascular component stress. This test was designed on human dermis microvascular endothelial cells (HDMEC). It enables us to assess the capability of an ingredient to regulate the skin’s reaction following vascular senescence stress, by monitoring specific markers.